Web31 May 2002 · It is proposed that a metal-binding motif involving the histidine residues within the sequence H411-X2-H414-X15-H430-X5-H436 is essential for gp17 function, which may be required for structural alignment and stabilization of functional sites in phage T4 terminase and other double-stranded DNA phage terminases. WebTo understand in molecular terms the terminase morphological change, we generated the terminase atomic model based on the crystallographic structure of its phage T4 counterpart. The docking of the threaded model in both terminase conformations showed that the transition between the two states can be achieved by rigid body subunit rotation in the …
Juan Carlos Sanchez - Myrtle Bank, South Australia, Australia ...
Web14 Nov 2024 · Phage PHB09 is highly homologous to Pseudomonas phage VCM based on the terminase large subunit, and this sequence was distinct from those of other groups (Figure 9a). Phylogenetic analysis using the sequences of the major capsid protein placed PHB09 on a separate branch, displaying a distant phylogenetic relationship to the … WebThe terminases subunits gp18 and gp19 select the right end of the concatemeric DNA thus initiating encapsidation. Once the unit-length DNA is translocated to the prohead, the terminase recognizes and cut the left end of the genome. After completion of packaging, the tail proteins cooperatively assemble on the filled head. . hcl share split
vB_BcM_Sam46 and vB_BcM_Sam112, members of a new …
WebL-terminase [8,9]. These functions are likely to be very important in vivo to sustain the enormous rate of genome packaging, which can be as high as ∼2000 bp/s[10]. ... In this phage, S-terminase assem-bles in solution and in crystals as a hollow nonamer [13–15],similartotheS-terminaseoftheSiphoviridae SPP1-likephageSf6 [16 ... Web7 Jun 2024 · Phage isolation and basic characterization. To the best of our knowledge, vB_EliS-R6L is the first phage isolated from the ecologically important marine bacteria of the genus Erythrobacter. vB_EliS-R6L forms small, clear, round (1–4 mm diameter) plaques on a bacterial lawn (Fig. 1).After treatment with different concentrations of chloroform (i.e., 1%, … WebEnter the email address you signed up with and we'll email you a reset link. gold coloured bangles