WebRNA work: RNA extraction, cDNA synthesis, Real-time PCR, in vitro transcription, Primer extension, RNA sequencing. DNA-Protein work: Electrophoretic Mobility Shift Assay, DNase 1 footprinting WebThere has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple, robust, inexpensive assay for quantitative analysis of …
Transcriptome characterisation and simple sequence repeat …
WebMar 29, 2024 · In vivo experiments proved that reducing ELF3 expression promoted the gemcitabine sensitivity of GBC cells and extended the survival time of mice that received orthotopic xenografted tumors. BACKGROUND Gemcitabine is the standard treatment for gallbladder cancer (GBC) patients, and the development of resistance frequently limits its … WebI also like to get my hands dirty in the wet lab (No bare hands, of course, proper personal protective equipment is a must!). My hands are great in small animal handling and husbandry, cell culture techniques (immortalized cells and primary culture), cell-based assays and molecular biology techniques covering from microRNA, RNA, DNA to protein. examples of start stop continue
Viruses Free Full-Text Development of a Novel RT-qPCR …
WebAug 18, 2004 · Two competing events, termination and readthrough (or nonsense suppression), can occur when a stop codon reaches the A-site of a translating ribosome. Translation termination results in hydrolysis of the final peptidyl-tRNA bond and release of the completed nascent polypeptide. Alternatively, readthrough, in which the stop codon is … WebIV. Primer Extension Reaction To assay the levels of a specific RNA, cDNA is synthesized using an end-labeled primer complementary to the target RNA. The amount of cDNA obtained is a measure of the amount of target RNA, and the size of the cDNA reflects the distance from the primer to the 5·-end of the RNA. WebApr 29, 2024 · In addition, an RNA primer extension assay was performed to verify whether the purified RdRp was active. The purified RdRp was incubated for 1 hour with a 40-mer … examples of starchy carbs