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Ph-ncas9-ppe-v2

http://enpcce.genetics.cas.cn/PN/CXG/SP/ WebbPlasmid pnCas9-PBE from Dr. Caixia Gao's lab contains the insert NLS-APOBEC1-XTEN-nCas9-UGI-NLS and is published in Nat Biotechnol. 2024 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2024 Feb 27. This plasmid is available through Addgene.

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Webb7 jan. 2024 · bacterial resistance : Kanamycin cloning : growth strain : We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion … WebbFor sgRNA expression in monocotyledons protoplasts. Loading... 170131. pH-nCas9-PPE-V2. For plant prime editing in rice plants or monocotyledons protoplasts. Loading... spyro reignited trilogy tree tops egg thief https://myfoodvalley.com

高效启动编辑与优化，配对pegRNAs在植物 自然生物技术beplay官 …

WebbThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. WebbpH-nCas9-PPE-V2 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated … WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended … sheriff pretoria north east

pH-nCas9-PPE-V2 Addgene 170131 product information

Category:Application of CRISPR-Based Technology in Plant Gene Editing …

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Ph-ncas9-ppe-v2

Selected Publications----State Key Laboratory of Plant Cell and ...

Webb24 mars 2024 · Engineered RTs, fusing functional elements or peptides to nCas9-RT (Cas9 nickase-reverse transcriptase), increasing the binding affinity of the nuclear localization signal sequences of nCas9... http://enpcce.genetics.cas.cn/PN/CXG/SP/

Ph-ncas9-ppe-v2

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Webb杭州宝赛生物是专业的质粒菌株公司，承接质粒进口，质粒构建，质粒改造，菌株基因编辑，菌株改造，合成生物学菌株构建服务。宝赛生物提供的质粒菌株产品，都有亲自实用经验提供，100%保证售后质量。宝赛生物可以提供 美国atcc，德国dsmz，美国nrrl，日本jcm，nbrc，英国ncimb，ncyc，荷兰cbs，瑞典 ... WebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, …

http://bio-sci.com.cn/index.php?catid=71&page=6 WebbpH-nCas9-PBE质粒价格1200元，pH-nCas9-PBE(爱迪基因 98163 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为卡那霉素。 pH-nCas9-PBE质粒是植物表达；水稻。

Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h. WebbpH-nCas9-PPE-V2. Plasmid #170131. Purpose. For plant prime editing in rice plants or monocotyledons protoplasts. Depositor. Caixia Gao. ... Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker.

WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. …

Webb24 juli 2024 · Product Name : pH-nCas9-PPE-V2 article : bacterial resistance : Kanamycin cloning : backbone pHUE411 (modified from pCambia) backbone_mutation … spyro reignited trilogy town squaresheriff pretoria north east contact numberWebbTotal vector size (bp) 6388 Vector type Plant Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Spectinomycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name BsaI:AATG:Cas9_2:GCTT:BsaI Species Synthetic Insert Size (bp) 4141 Tag / Fusion Protein sheriff pretoria north west contact details