WebThis tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission … WebAcquired cell suspension was filtered, lysed with ACK Lysis Buffer, stained with FVS780- APC-CY7 and Human Fc block. Afterwards, cells were stained with anti-human …

BD Biosciences Fluorochrome Reference Chart

WebSep 19, 2012 · This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link ® APC/Cy7 Labeling Kit. 765-0015 is the same as the 1 mg size. 765-0010 is the same as the 3 x 100 ug size. 765-0030 is the same as the 3 x 10 ug size. 765-0005 is the same as the 100 µg size.. Amount and volume of antibody for … WebFixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak emission of 780 nm that can be detected using a … round flag cake

BD Horizon™ Fixable Viability Stain (FVS) Reagents

WebBring FVS780 dye powder and 180 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. Add 180 ... be read out of filters commonly used … WebAPC-Cy7, or CD8 APC-Cy7. Stained samples were stored in 1% PFA at room temperature under ambient light for 48 hours. BD APC-H7 vs. APC-Cy7: Comparison of Staining Stability (in BDSF, under light at RT) 0 50 100 150 200 250 0 5 10 15 20 25 30 35 40 45 50 CD4 APC-H7 Time (hour) CD8 APC-H7 WebAPC-Cy7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter … strathmore cfa