Fluxor assay
WebScreening and assay development in a high throughput screening department; - Executed fluorescence, beta-lactamase and radioactive High Throughput Screens; focus on Calcium flux and membrane... WebThe FluxOR™ assay is an alternative to one form of an established screening method for K+ channels using radioactive rubidium, which is highly radioactive and banned from …
Fluxor assay
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WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … WebFluxOR Potassium Ion Channel Assay BacMam Ion Channels The BacMam system has been used to create K+ and Na+ ion channel reagents which can be transduced into mammalian cells with ease. This portability allows for faster assay development in pharmacologically relevant cell types. The BacMam System
WebMay 14, 2024 · FluxOR Assay Renal outer medullary potassium channel activity was assessed by a fluorescence-based microplate assay (FluxOR II Green Potassium Ion Channel Assay). The assay is based on the fact that thallium influx serves as a surrogate for K + channel and transporter activity and takes advantage of recently developed …
WebAug 8, 2024 · Following the manufacturer’s procedure, which requires supplementation of assay and stimulus buffers with a proprietary background suppressor, FluxOR Red exhibited a 5.37 ± 0.001-fold increase in fluorescence at the highest concentration of ML297 compared to Thallos (4.30 ± 0.008-fold increase) and 8c (3.09 ± 0.004-fold increase). WebFluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel...
WebJul 29, 2024 · After 30 min of ATP stimulation (A) intracellular levels of K + was measured by the FluxOR II green potassium ion channel assay. BMDMs were either left untreated (0 mM) or treated with increasing concentrations of extracellular KCL (10 mM, 20 mM and 40 mM) and then infected with different CDC1551 Mtb strains (Mtb, ΔpknF mutant and …
WebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … sharif small baltimoreWebFluxOR™ hERG Assay. Background and Service Details: hERG (human Ether-a-go-go-Related Gene, KCNH2) is a gene encoding alpha-subunit of a potassium channel. Properly formed and functional potassium channels are essential for normal electrical activity in the heart. Interference with the potassium channel due to the drug inhibition can result ... sharif solimanWebFluxor comes with two implementations of Interceptor: PrintInterceptor for printing Actions and state changes to the log. TestInterceptor to help assert that specific Actions was … poppin lightWeb2 days ago · Magnetic structure: Fluxor magnet array. Magnet type: Neodymium N50. Phase management: Fazor. Earpads: Synthetic leather and gel filling. Weight: 375g. … sharif solicitorsWebFluxOR™ II Green Potassium Ion Channel Assay 2 Product information The FluxOR™ II Green Potassium Ion Channel Assay is a solution for high-throughput screening (HTS) … poppin lockersWebMar 17, 2024 · The FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit … poppin light fontWebA library of 1280 biologically active compounds from the Tocris catalog. Covers a wide range of pharmacological targets and research areas. Compounds are supplied pre-dissolved in 250 μ L 10 mM DMSO solutions. A smaller volume is available as Tocriscreen Plus Mini (Cat. No. 5841). Key Format and Product Details for Tocriscreen Plus poppin lyrics big scarr