WebThe HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. WebConfocal immunofluorescent analysis of mouse spleen (left, positive) and liver (right, negative) using CD45R/B220 (RA3-6B2) Rat mAb (FITC Conjugate) #34399 (green). Actin filaments were labeled with DyLight ™ …
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WebThe cells were fluorescently stained with CD45R (B220)-APC, CD19-FITC, and CD138-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and … WebFluorescein isothiocyanate (FITC, Sigma) was used as a fluorescent dye to label MBG NPs for the immune escape analysis in vitro. ... (558295, BD), CD3e v450 (560801, BD), and B220 PE (553089, BD), and analysis by flow cytometry. 2.5. In vitro and in vivo tumor-targeted effect ...
WebDec 15, 2002 · Total spleen cells were stained with anti-CD11c-FITC, -B220-APC, and -CD19-PE mAbs. An electronic gate was performed on CD19 − cells to exclude B cells, and CD11c vs B220 expression was analyzed. Numbers indicate the percentage of cells for each region among CD19 − cells. WebJan 10, 2024 · Alexa Fluor® dyes are a big group of negatively charged and hydrophilic fluorescent dyes, frequently used in fluorescence microscopy. All the Alexa Fluor® dyes are sulfonated forms of different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamine (e.g. Alexa Fluor®546, Alexa Fluor®633).The respective laser …
Web1 day ago · Chronic liver disease is a major public health burden worldwide1. Although different aetiologies and mechanisms of liver injury exist, progression of chronic liver disease follows a common pathway ... WebSep 30, 2024 · c Freshly purified B cells from WT and V154M mice were surface stained with IgM-Alexa 568, with B220-BV605 and IgD-FITC, or with CD19-Alexa 647 and Igβ-FITC. Gated live cells were analyzed for ...
WebThe FITC Anti-Human/Mouse CD45R (B220) (RA3-6B2) Antibody from Tonbo Biosciences is a Rat Monoclonal antibody to PTPRC. This antibody recognizes Human, and Mouse …
WebCD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. candy melts woolworthsWebCD45R (B220) Monoclonal Antibody (RA3-6B2), FITC, eBioscience™ ... CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. candy melts milk chocolateWebApr 1, 2010 · To obtain the percentage of different lineage of blood cells in bone marrow, cells were flushed and filtered to get single-cell suspensions. A cocktail of antibodies (Mac-1 APC, Gr-1 PE, Ter119 FITC, B220 FITC, CD3 APC, CD4 FITC, CD8 PE) were used to stain the cells. The percentage of gated cells was obtained by flow cytometry. fishwick motors herefordRequest Bulk Quote. Description. CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. fishwick rangersWebFITC conjugate, Type IV, lyophilized powder. View Price and Availability. Sigma-Aldrich. B2261. bisBenzimide H 33342 trihydrochloride. ≥98% (HPLC and TLC) ... with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs) to visualize the cone outer segments of pre-treated retinal sections; Packaging. 1, 2, 5 mg in glass bottle. candy mercier obituaryWebProduct Specific Information. Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell … fishwick preston mapWebWe would like to show you a description here but the site won’t allow us. candy melts lip balm price