Cytolight rapid red
WebIncucyte® NucLight Rapid Red Reagent for live-cell nuclear labeling by Sartorius Group Manufacturer Sartorius Group Model: 4717 2.3 / 5.0 1 … WebTo discriminate effector cells from target cells, effector cells were labeled with CTV, as per the manufacturer’s instructions, prior to the co-culture assays. For live-cell IncuCyte imaging cytotoxicity assay, effector cells were labeled with CytoLight rapid green (Sartorius, Göttingen, Germany), as per the manufacturer’s instructions.
Cytolight rapid red
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WebIncuCyte® CytoLight Reagents They are a unique range of lentiviral-based labeling reagents with simple transduction protocols that enable expression of a cytoplasmically soluble green (GFP) or red (mKate2) fluorescent … WebDec 15, 2024 · Further, the Incucyte ® Cytolight Rapid Red or Green regents can be employed to assess T cell killing and antibody-dependent cell-mediated cytotoxicity (ADCC assays), respectively. These dyes are ideal for identifying the location of cells within a population and monitoring cellular interactions (Fig. 3). They freely pass through cell ...
WebFeatures: Efficient, non-perturbing nuclear labeling of live mammalian cells for cell counting Homogenous expression of a nuclear-restricted mKate2 (red fluorescent protein) Minimal toxicity, no alteration of cell function Ideal for generating stable cell populations or clones using puromycin selection WebIncucyte® NucLight Red Lentivirus Reagent (EF1α, Puro) - 0.2mL by Sartorius Group Manufacturer Sartorius Group Model: 4625 Be the first to review this product One vial of Lentivirus reagent (0.2 mL; >1x106 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted red fluorescent label.
WebIncucyte® CytoLight Rapid Red Reagent for live cell cytoplasmic labeling by Sartorius Group. Manufacturer Sartorius Group Model: 4706. WebThe Incucyte ® Cell Cycle Green/Red Lentivirus Reagent is a fluorescent, single cassette indicator expressing both the GFP (green fluorescent protein) and mKate2 (red fluorescent protein) to distinguish between cells in the G1 and S/G2/M cell cycle phase without altering cell function.. This reagent is ideal for generating stable cell populations or clones using …
Webwith the IncuCyte live-cell analysis system and describes how IncuCyte Nuclight Rapid Red can be used to fluorescently label the nucleus of living cells without perturbing cell function or biology. In addition, this reagent … graph productWebSep 30, 2024 · Additionally, primary human nucleus pulposus cells (NPCs) of the IVD were stained with IncuCyte® CytoLight Rapid Red reagent (cat. No. 4706). These cells were then seeded in 96-well plates in a ‘race’ experiment seeding 50:50 with 4000 cells per cell type, and the interactions and the cell proliferation between the two cells was monitored ... chi st alexius dickinson ndWebMAXIMUS. May 2002 - Mar 20052 years 11 months. McLean, VA. Mr. Sanderson created compliant and winning services proposals based on State, local and Federal RFP’s … chi st alexius dickinson nd fax numberWebWe optimized IncuCyte® CytoLight Rapid Green Reagent for live cell cytoplasmic labeling with Jurkat cells, pmel-1 T cells, and IncuCyte® CytoLight Rapid Red Reagent with B16F10 melanoma cells. The optimal concentrations (based on minimal decrease in cell viability and highest percentage of stained cells) were: Jurkat: 1 µM (green) graph program in c++WebMay 19, 2024 · CellTrace™ CFSE and CellTrace™ Violet were obtained from Thermo Fisher Scientific (Cleveland, OH, USA) and Incucyte ® Cytolight Rapid Red Dye for Live-Cell Cytoplasmic Labeling (Cytolight Red) from Sartorius (Göttingen, Germany). 1,10-dioctadecyl-3,3,30,30–tetramethylindodicarbocyanine (DiD) was obtained from Invitrogen … chi st alexius bismarck rheumatologyWebOct 3, 2016 · The alkaloid cytochalasin D is recognized as a potent inhibitor of F-actin polymerization.1 When cytochalasin D is present in higher concentrations, multiple axon-like structures are shown to be... chi st. alexius healthWebIncucyte ® Cytolight Rapid Green Dye 2QHYLDO J 4705 SX5, S3, SX1 Incucyte ® Cytolight Rapid Red Dye )LYHYLDOV J 4706 6; FRQ®JXUHGZLWK*UHHQ 5HG Optical Module), S3, SX1 Cytoplasmic Lentivirus Labeling Reagents Lentivirus reagents provide homogenous expression of a fluorescent protein without altering cell function for live-cell chi st alexius health devils lake nd