WebWhen changing the volume injecting the peaks doesn’t change. I tried a new column, using HPLC grade water, cleaning the LC system (several time), using another HPLC, changing the injector,... WebAug 28, 2024 · The presence of three sets of peaks means that there are three different chemical environments that the hydrogens can be found in: the terminal methyl (CH 3) …

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WebYou need a much stronger solvent to clean the column. Try flush it with 5% water/acetonitrile at the end of the day. Check by looking at the chromatogram of blank eluted with this solvent. If you... WebUse clean, particle‐free solvents When preparing mobile phase, always use chemically clean and particle‐free solvents (Table 3 ) and reagents. Solvent must be UPLC‐grade or a quality level suitable to the application being run. (See Solvent considerations, page 23.) Use fresh solvents boomerang catapult traverse city

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