WebMay 21, 2024 · A Back-splicing driven by the pairing of intronic complementary sequences, RNA-binding protein (RBP), or lariat structure containing skipped exons or introns. B … WebDec 1, 2024 · CircRNAs are widely present in eukaryotes, which are mostly generated through the back-splicing of pre-mRNAs. Diverse regulatory functions of circRNAs were …
Knockout of circRNAs by base editing back-splice sites of …
WebNov 19, 2024 · In Fawn Creek, there are 3 comfortable months with high temperatures in the range of 70-85°. August is the hottest month for Fawn Creek with an average high … WebMar 10, 2024 · The splicing factor musclebind (MBL/MBNL1) strongly and specifically binds to circMbl, a circular form that is generated from the second exon of its own mRNA; this interaction increases circRNA levels in dependance on the binding of MBL on intronic consensus sites flanking the exon [ 55 ]. biocia healthcare
The m6A reader YTHDC1 and the RNA helicase DDX5 control the …
We first sought to uncover principles behind circRNA vector topology that are necessary for strong translation. We began by synthesizing circRNAs with a coxsackievirus B3 (CVB3) IRES (denoted iCVB3) downstream, or 3′, of the reporter NanoLuc gene, maintaining the reading frame through the … See more Synthesis of circRNAs via intron-assisted splicing and RNaseR digestion has been previously described4, but rapid creation of different circRNA species was difficult. To enable higher-throughput testing of circRNAs, we … See more Viral IRESs are diverse and highly structured RNA regions found primarily in viral 5′ UTRs that promote cap-independent translation30,31,32. Because iCVB3, the baseline … See more We previously showed that circRNAs can trigger immune responses in vivo that can be avoided by modifying circRNAs with m6A4,11. However, … See more 5′ and 3′ UTRs in mRNAs can recruit RNA-binding proteins (RBPs) that enable strong translation as well as aspects of post-transcriptional regulation6. One such family of RBPs is poly(A)-binding proteins (PABPs), which … See more WebAug 12, 2024 · Back-splicing is the key step in circRNA formation. In this study, we propose circCNN to predict the back-splicing sites of CircRNA formation. Unlike … WebJul 15, 2024 · CircRNAs are generated by a “back-splicing” process, which is regulated by different cis-regulatory elements and trans-acting proteins. Therefore, how these cis and trans elements change during tumorigenesis and how they regulate the biogenesis of circRNAs in cancer are two questions that interest us. dagmar thorpe